用洋葱球茎提取物治疗小鼠哮喘模型可预防和逆转过敏性炎症
2024/10/29
摘要
背景：哮喘是一项全球性的健康挑战。主要的药物疗法是化学合成药物和生物制剂，这些药物成本高，副作用大。与此相反，由于洋葱（Allium cepa L., Amaryllidaceae）等天然产品疗效显著，且几乎不存在安全问题，因此全世界越来越多地使用它们来治疗气道疾病。然而，它们的药理作用在很大程度上仍未得到定性。我们研究了洋葱球茎提取物（OBE）是否能（1）逆转已确定的哮喘表型（治疗性治疗）和/或（2）在免疫过程前服用（预防性治疗），防止哮喘表型的发展。
方法：在治疗方案（21 天）和预防方案（19 天）中，分别设立了六组雄性 Balb/c 小鼠和五组雄性 Balb/c 小鼠；其中包括安慰剂或 OBE（30、60 和 100 mg/kg/i.p.）治疗的 PBS 小鼠和经屋尘螨（HDM）感染的小鼠。气道炎症通过细胞学、组织学、免疫荧光、Western 印迹和血清 IgE 进行检测。
结果：治疗性（60 mg/kg/i.p.）和预防性（100 mg/kg/i.p.）OBE 治疗可下调 HDM 诱导的气道细胞流入、组织病理学变化以及促炎信号通路 EGFR、ERK1/2、AKT、促炎细胞因子和血清 IgE 的表达。
结论：我们的研究结果表明，OBE 是一种有效的抗炎药物，具有治疗和预防哮喘的作用。这些研究结果表明， OBE 可作为哮喘的辅助治疗药物和/或预防过敏性哮喘的发生。不过，还需要进一步研究以确定其活性成分，并在人体中证明。
Treatment with onion bulb extract both prevents and reverses allergic inflammation in a murine model of asthma
El-Hashim AZ, Khajah MA, Orabi KY, Balakrishnan S, Sary HG, Barakat AM.
Abstract
Background:Asthma presents a global health challenge. The main pharmacotherapy is synthetic chemicals and biological-based drugs that are costly, and have significant side effects. In contrast, use of natural products, such as onion (Allium cepa L., Amaryllidaceae) in the treatment of airway diseases has increased world-wide because of their perceived efficacy and little safety concerns. However, their pharmacological actions remain largely uncharacterized. We investigated whether onion bulb extract (OBE) can (1) reverse established asthma phenotype (therapeutic treatment) and/or (2) prevent the development of the asthma phenotype, if given before the immunization process (preventative treatment).
Methods:Six groups of male Balb/c mice were established for the therapeutic (21 days) and five groups for the preventative (19 days) treatment protocols; including PBS and house dust mite (HDM)-challenged mice treated with vehicle or OBE (30, 60, and 100 mg/kg/i.p.). Airways inflammation was determined using cytology, histology, immunofluorescence, Western blot, and serum IgE.
Results:Therapeutic (60 mg/kg/i.p.) and preventative (100 mg/kg/i.p.) OBE treatment resulted in down-regulation of HDM-induced airway cellular influx, histopathological changes and the increase in expression of pro-inflammatory signaling pathway EGFR, ERK1/2, AKT, pro-inflammatory cytokines and serum IgE.
Conclusion:Our data show that OBE is an effective anti-inflammatory agent with both therapeutic and preventative anti-asthma effects. These findings imply that onion/OBE may be used as an adjunct therapeutic agent in established asthma and/or to prevent development of allergic asthma. However, further studies to identify the active constituents, and demonstrate proof-of-concept in humans are needed.
背景：哮喘是一项全球性的健康挑战。主要的药物疗法是化学合成药物和生物制剂，这些药物成本高，副作用大。与此相反，由于洋葱（Allium cepa L., Amaryllidaceae）等天然产品疗效显著，且几乎不存在安全问题，因此全世界越来越多地使用它们来治疗气道疾病。然而，它们的药理作用在很大程度上仍未得到定性。我们研究了洋葱球茎提取物（OBE）是否能（1）逆转已确定的哮喘表型（治疗性治疗）和/或（2）在免疫过程前服用（预防性治疗），防止哮喘表型的发展。
方法：在治疗方案（21 天）和预防方案（19 天）中，分别设立了六组雄性 Balb/c 小鼠和五组雄性 Balb/c 小鼠；其中包括安慰剂或 OBE（30、60 和 100 mg/kg/i.p.）治疗的 PBS 小鼠和经屋尘螨（HDM）感染的小鼠。气道炎症通过细胞学、组织学、免疫荧光、Western 印迹和血清 IgE 进行检测。
结果：治疗性（60 mg/kg/i.p.）和预防性（100 mg/kg/i.p.）OBE 治疗可下调 HDM 诱导的气道细胞流入、组织病理学变化以及促炎信号通路 EGFR、ERK1/2、AKT、促炎细胞因子和血清 IgE 的表达。
结论：我们的研究结果表明，OBE 是一种有效的抗炎药物，具有治疗和预防哮喘的作用。这些研究结果表明， OBE 可作为哮喘的辅助治疗药物和/或预防过敏性哮喘的发生。不过，还需要进一步研究以确定其活性成分，并在人体中证明。
(中日友好医院呼吸与危重症医学科 沈焜路 摘译 林江涛 审校)
(Pharm Biol. 2024 Dec; DOI: 10.1080/13880209.2024.2335187)
(Pharm Biol. 2024 Dec; DOI: 10.1080/13880209.2024.2335187)
Treatment with onion bulb extract both prevents and reverses allergic inflammation in a murine model of asthma
El-Hashim AZ, Khajah MA, Orabi KY, Balakrishnan S, Sary HG, Barakat AM.
Abstract
Background:Asthma presents a global health challenge. The main pharmacotherapy is synthetic chemicals and biological-based drugs that are costly, and have significant side effects. In contrast, use of natural products, such as onion (Allium cepa L., Amaryllidaceae) in the treatment of airway diseases has increased world-wide because of their perceived efficacy and little safety concerns. However, their pharmacological actions remain largely uncharacterized. We investigated whether onion bulb extract (OBE) can (1) reverse established asthma phenotype (therapeutic treatment) and/or (2) prevent the development of the asthma phenotype, if given before the immunization process (preventative treatment).
Methods:Six groups of male Balb/c mice were established for the therapeutic (21 days) and five groups for the preventative (19 days) treatment protocols; including PBS and house dust mite (HDM)-challenged mice treated with vehicle or OBE (30, 60, and 100 mg/kg/i.p.). Airways inflammation was determined using cytology, histology, immunofluorescence, Western blot, and serum IgE.
Results:Therapeutic (60 mg/kg/i.p.) and preventative (100 mg/kg/i.p.) OBE treatment resulted in down-regulation of HDM-induced airway cellular influx, histopathological changes and the increase in expression of pro-inflammatory signaling pathway EGFR, ERK1/2, AKT, pro-inflammatory cytokines and serum IgE.
Conclusion:Our data show that OBE is an effective anti-inflammatory agent with both therapeutic and preventative anti-asthma effects. These findings imply that onion/OBE may be used as an adjunct therapeutic agent in established asthma and/or to prevent development of allergic asthma. However, further studies to identify the active constituents, and demonstrate proof-of-concept in humans are needed.
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