补充牛磺酸可减轻因次氯酸暴露而加重的哮喘气道炎症
2025/04/06
摘要
环境污染物暴露已被证实与哮喘的发生和进展相关。次氯酸作为一种环境暴露相关的氯基消毒剂，其在哮喘气道炎症中的作用尚不清楚。通过在生命早期和围产期给予次氯酸饮水，我们发现生命早期次氯酸饮水暴露不仅加重了哮喘小鼠的气道炎症，而且围产期次氯酸饮水暴露可促进哮喘小鼠子代的气道炎症。通过肠道菌群测序发现，次氯酸饮水暴露可降低哮喘小鼠肠道菌群多样性，乳杆菌属、厚壁菌属、毛螺菌属和优杆菌属的丰度升高。进一步的粪便代谢组学分析显示，次氯酸饮水暴露显著增强了花生四烯酸代谢通路。显著改变菌属的丰度与花生四烯酸代谢物水平之间存在一定的相关性。最后，次氯酸中和剂牛磺酸治疗表明，牛磺酸可以显著减轻次氯酸暴露导致的哮喘气道炎症。综上所述，这些结果为次氯酸暴露加重哮喘气道炎症提供了证据，并证实补充牛磺酸可作为一种潜在的治疗方法。
Taurine supplementation alleviates asthma airway inflammation aggravated by HOCl exposure
Yingjun Chen, Shiting Yi, Qian Wang, Yanwen Li, Sitong Lin, Shixiu Liang
Abstract
Environmental pollutant exposure has been demonstrated to be associated with the onset and progression of asthma. Hypochlorous acid (HOCl), as an environmental exposure-relevant chlorine-based disinfectant, its role in asthmatic airway inflammation remains unclear. Through administering HOCl in drinking water during early life and the perinatal period, we discovered that early-life HOCl drinking water exposure not only aggravated airway inflammation in asthmatic mice but also that perinatal HOCl drinking water exposure could promote airway inflammation in the offspring of asthmatic mice. By gut microbiota sequencing, it was found that HOCl drinking water exposure could reduce the gut microbiota diversity in asthmatic mice, with the abundances of Lactobacillus, Faecalibaculum, Muribaculum, and [Eubacterium]_ventriosum_group being decreased, while increasing the abundances of Dubosiella and Parabacteroides. Further fecal metabolomics analysis revealed that HOCl drinking water exposure significantly enhanced the arachidonic acid metabolism pathway. And there was a certain correlation between the abundances of the significantly altered bacterial genera and the levels of arachidonic acid metabolites. Finally, treatment with taurine, a HOCl neutralizer, showed that taurine could significantly alleviate the asthma airway inflammation aggravated by HOCl exposure. In summary, these results provide evidence for the exacerbation of asthma airway inflammation by HOCl exposure and confirm that taurine supplementation can serve as a potential therapeutic approach.
环境污染物暴露已被证实与哮喘的发生和进展相关。次氯酸作为一种环境暴露相关的氯基消毒剂，其在哮喘气道炎症中的作用尚不清楚。通过在生命早期和围产期给予次氯酸饮水，我们发现生命早期次氯酸饮水暴露不仅加重了哮喘小鼠的气道炎症，而且围产期次氯酸饮水暴露可促进哮喘小鼠子代的气道炎症。通过肠道菌群测序发现，次氯酸饮水暴露可降低哮喘小鼠肠道菌群多样性，乳杆菌属、厚壁菌属、毛螺菌属和优杆菌属的丰度升高。进一步的粪便代谢组学分析显示，次氯酸饮水暴露显著增强了花生四烯酸代谢通路。显著改变菌属的丰度与花生四烯酸代谢物水平之间存在一定的相关性。最后，次氯酸中和剂牛磺酸治疗表明，牛磺酸可以显著减轻次氯酸暴露导致的哮喘气道炎症。综上所述，这些结果为次氯酸暴露加重哮喘气道炎症提供了证据，并证实补充牛磺酸可作为一种潜在的治疗方法。
(中日友好医院呼吸与危重症医学科 顾宪民 摘译 林江涛 审校)
Taurine supplementation alleviates asthma airway inflammation aggravated by HOCl exposure
Yingjun Chen, Shiting Yi, Qian Wang, Yanwen Li, Sitong Lin, Shixiu Liang
Abstract
Environmental pollutant exposure has been demonstrated to be associated with the onset and progression of asthma. Hypochlorous acid (HOCl), as an environmental exposure-relevant chlorine-based disinfectant, its role in asthmatic airway inflammation remains unclear. Through administering HOCl in drinking water during early life and the perinatal period, we discovered that early-life HOCl drinking water exposure not only aggravated airway inflammation in asthmatic mice but also that perinatal HOCl drinking water exposure could promote airway inflammation in the offspring of asthmatic mice. By gut microbiota sequencing, it was found that HOCl drinking water exposure could reduce the gut microbiota diversity in asthmatic mice, with the abundances of Lactobacillus, Faecalibaculum, Muribaculum, and [Eubacterium]_ventriosum_group being decreased, while increasing the abundances of Dubosiella and Parabacteroides. Further fecal metabolomics analysis revealed that HOCl drinking water exposure significantly enhanced the arachidonic acid metabolism pathway. And there was a certain correlation between the abundances of the significantly altered bacterial genera and the levels of arachidonic acid metabolites. Finally, treatment with taurine, a HOCl neutralizer, showed that taurine could significantly alleviate the asthma airway inflammation aggravated by HOCl exposure. In summary, these results provide evidence for the exacerbation of asthma airway inflammation by HOCl exposure and confirm that taurine supplementation can serve as a potential therapeutic approach.
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