哮喘联盟

   摘要
   背景：通过对哮喘病情的控制试验对哮喘的严重程度进行评估是近年来较为常用的手段。但是，目前还没有一种全身炎症反应的生物标志物用以评估哮喘的控制情况。
   目的：本研究旨在测定在哮喘儿童血浆中对氧磷酸酶活性（PON1）、总的氧化剂水平（TOS）及总的抗氧化水平（TAS），并评估以上指标在哮喘控制及识别哮喘发作患者中的作用。
   方法：85例病情在控制中的哮喘儿童及55例健康儿童参加了本项研究。采集其血液标本用以测定血浆对氧磷酸酶活性（PON1）、总的氧化剂水平（TOS）及总的抗氧化水平（TAS）等指标。所有可能影响以上三项指标检测的因素均被排除。该三项指标的潜在诊断性能通过受试者操作特征曲线（ROC曲线）来评估。
   结果：哮喘儿童与健康儿童的检测结果表明，哮喘儿童血浆中总的抗氧化水平（6.9±2.1 vs. 1.05±0.32，P<0.001）及总的氧化剂水平（12.5±3.4 vs. 5.5±3.8，P<0.001）显著高于健康儿童组，而其血浆对氧磷酸酶活性却显著低于健康儿童组（156.5±55 vs. 298.6±87.6，P<0.001）。ROC分析结果表明，血浆对氧磷酸酶活性及总的氧化剂水平对于诊断哮喘发作的曲线下面积分别为0.679和0.645。而哮喘控制试验（ACT）对于诊断哮喘发作的曲线下面积为0.972。
   结论：血浆对氧磷酸酶活性及总的氧化剂水平可能可以作为哮喘发作的系统性评估指标。结合使用哮喘控制试验、血浆对氧磷酸酶活性及总的氧化剂水平可诊断哮喘儿童是否处于发作期。


 

（苏楠 审校）
AllergolImmunopathol(Madr).2014Oct8.pii:S0301-0546(14)00136-0.doi:10.1016/j.aller.2014.05.009. [Epub ahead of print]


 

Plasma paraoxonase, oxidative status level, and their relationship with asthma control test in children with asthma.
 

Emin O1, Hasan A2, Rusen DM3.
 

ABSTRACT
BACKGROUND: Assessment of asthma with a control test has been suggested as a relevant approach in recent years. However, no biomarker of systemic inflammation has been included in the assessment of asthma control.
OBJECTIVE: To evaluate plasma paraoxonase (PON1), total oxidant status (TOS), and total antioxidant status (TAS) levels in children with asthma according to the disease control, and the performance in the identification of uncontrolled patients.
METHODS: Stable asthmatic children (n=85) and healthy controls (n=55) were recruited for this study. Blood samples were collected for plasma PON1, TOS, and TAS measurements. Any contributing factors that may affect plasma PON1, TAS, and TOS levels were excluded from both groups. The diagnostic potential of these measures was evaluated using receiver operating characteristic (ROC) analysis.
RESULTS: Comparing the asthmatic children with the control group, plasma TAS and TOS levels were significantly higher (TAS; 6.9±2.1, 1.05±0.32, P<0.001, and TOS; 12.5±3.4, 5.5±3.8, P<0.001, respectively) and PON1 level was significantly lower (156.5±55, 298.6±87.6, respectively, P<0.001) in the asthmatic group than controls. In ROC analysis, PON1 presented an AUC 0.679 and TOS presented an AUC 0.645 for the identification of uncontrolled asthma, respectively. Asthma Control Test (ACT) presented an AUC of 0.972 for the identification of uncontrolled asthma.
CONCLUSION: PON1 and TOS levels may be systemic markers of uncontrolled asthma in children. Combined use of these two biomarkers with asthma control test may identify patients with uncontrolled asthma in children.
 

AllergolImmunopathol(Madr).2014Oct8.pii:S0301-0546(14)00136-0.doi:10.1016/j.aller.2014.05.009. [Epub ahead of print]